Active Recombinant Human Protease Protein, 6×His tagged

• Cat.No.: Protease-01
• Product Overview: Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins made with SUMOstar expression systems. Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar. As a result, SUMOstar Protease 1 will not cleave within the protein of interest. SUMOstar Protease 1 will not cleave SUMO-tags from proteins expressed with the SUMOpro or SUMOpro3 systems. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1 and to cleave SUMOpro3 tag please use SUMO Protease 2.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Form: Liquid
• Bio-activity: 1 U will cleave > 90 μg of SUMOstar-GFP in 1 hr at 37 centigrade.
• Molecular Mass: 26.4 kDa
• Purity: > 95% by SDS-PAGE and RP-HPLC
• Applications: Removal of SUMOstar tags from recombinant, fusion proteins.
• Storage: At -80 centigrade, avoid freeze/thaw cycles.
• Storage Buffer: 50 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol

Gene Information

• Official Symbol: Protease
• Synonyms: Protease