Source : |
HEK293 |
Species : |
Human |
Tag : |
His |
Form : |
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized human CD155 at 2 μg/mL (100 μL/well) can bind human DNAM1, the EC50 of human DNAM1/CD226 is 50-300 ng/mL. |
Molecular Mass : |
The recombinant human CD155 consists of 334 amino acids and has a predicted molecular mass of 36.5 kDa. As a result of glycosylation, the rhCD155/Fc monomer migrates as approximately 60-65 kDa band in SDS-PAGE under reducing conditions. |
Protein Length : |
Met1-Asn343 |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 97 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |