Active Recombinant Human QSOX1 protein, His-tagged

• Cat.No.: QSOX1-285H
• Product Overview: Recombinant Human QSOX1(Ser33-Ala546) fused with an N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Description: Sulfhydryl Oxidase-1 (QSOX1) is an approximately 80 kDa enzyme that contains thioredoxin and sulfhydryl oxidase domains. It is synthesized with a C-terminal transmembrane segment, but soluble secreted forms can be generated by alternative splicing or proteolytic shedding within the Golgi. Within the region encompassing both enzymatic domains and the central region, human QSOX1 shares 79% aa sequence identity with mouse and rat QSOX1. It plays a role nascent protein folding by mediating disulfide oxidation. This activity is required for Laminin incorporation into the extracellular matrix. QSOX1 is up-regulated in many cancers and supports tumor cell proliferation and invasion.
• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to produce hydrogen peroxide during the oxidation of Dithiothreitol (DTT). The specific activity is >1,200 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass: 58 kDa
  SDS-PAGE: 55 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: QSOX1 quiescin Q6 sulfhydryl oxidase 1 [ Homo sapiens ]
• Official Symbol: QSOX1
• Synonyms: QSOX1; quiescin Q6 sulfhydryl oxidase 1; QSCN6, quiescin Q6; sulfhydryl oxidase 1; hQSOX; Q6; QSCN6; FLJ34858
• Gene ID: 5768
• mRNA Refseq: NM_001004128
• Protein Refseq: NP_001004128
• MIM: 603120
• UniProt ID: O00391