Active Recombinant Human ROBO1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ROBO1-042H
• Product Overview: Biotinylated Recombinant Human ROBO1 protein(GIn 26 - Pro 897), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: GIn 26 - Pro 897
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Anti-ROBO1 Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human ROBO1, His,Avitag with a linear range of 2-31 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 99.2 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: ROBO1 roundabout, axon guidance receptor, homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: ROBO1
• Synonyms: ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout (axon guidance receptor, Drosophila) homolog 1; roundabout homolog 1; DUTT1; FLJ21882; SAX3; deleted in U twenty twenty; MGC131599; MGC133277
• Gene ID: 6091
• mRNA Refseq: NM_001145845
• Protein Refseq: NP_001139317
• MIM: 602430
• UniProt ID: Q9Y6N7