Active Recombinant Human RSPO2 Protein, His-tagged

• Cat.No.: RSPO2-866H
• Product Overview: Recombinant Human RSPO2 (Accession # NP_848660) Gln22-Gly205, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Gln22-Gly205
• Predicted N Terminal: No results obtained: Gln22 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to synergize with Recombinant Mouse Wnt-3a to stabilize beta -catenin protein levels in HEK293T human embryonic kidney cells as assessed with Western blot.This effect is detected at doses as low as 1 ng/mL in the presence of 20 ng/mL recombinant mouse Wnt-3a.Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The typical ED50 is 0.7-2.8 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
• Molecular Mass: Recombinant Human RSPO2, His-tagged has a calculated MW of 22 kDa. In SDS-PAGE migrates as 25-29 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: RSPO2 R-spondin 2 [ Homo sapiens ]
• Official Symbol: RSPO2
• Synonyms: RSPO2; R-spondin 2; R spondin 2 homolog (Xenopus laevis); R-spondin-2; MGC35555; R-spondin 2 homolog; roof plate-specific spondin-2; CRISTIN2; MGC43342
• Gene ID: 340419
• mRNA Refseq: NM_178565
• Protein Refseq: NP_848660
• MIM: 610575
• UniProt ID: Q6UXX9
• Chromosome Location: 8q23.1