Active Recombinant Human SDC3 Protein, His-tagged

• Cat.No.: SDC3-685H
• Product Overview: Recombinant Human SDC3 (Accession # NP_055469) Gln48-Lys383, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Gln48-Lys383
• Predicted N Terminal: No results obtained: Gln48 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to inhibit the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin.When 5 x 10^4 cells/well are added to human Syndecan-3 and human Fibronectin (0.5 µg/mL) coated plates, cell adhesion is blocked in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 2.5 - 10 µg/mL.
• Molecular Mass: Recombinant Human SDC3, His-tagged has a calculated MW of 35.5 kDa. In SDS-PAGE migrates as 100-150 kDa, reducing conditions.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SDC3 syndecan 3 [ Homo sapiens ]
• Official Symbol: SDC3
• Synonyms: SDC3; syndecan 3; syndecan 3 (N syndecan); syndecan-3; N syndecan; SYND3; syndecan proteoglycan 3; N-syndecan; syndecan neural type; SDCN
• Gene ID: 9672
• mRNA Refseq: NM_014654
• Protein Refseq: NP_055469
• MIM: 186357
• UniProt ID: O75056
• Chromosome Location: 1pter-p22.3