Active Recombinant Human SERPINA4 protein, His-tagged

• Cat.No.: SERPINA4-4532H
• Product Overview: Active Recombinant Human SERPINA4 protein(P29622)(21-427 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 21-427 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Kallistatin (KAL), a member of serpin family, can Inhibit human amidolytic and kininogenase activities of tissue kallikrein. KAL is widely distributed in a variety of organs and body fluids and has biological effects such as anti-inflammatory, antioxidant stress, anti-angiogenesis and anti-tumor. Besides, Kallikrein 2 (KLK2) has been identified as an interactor of KAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human KAL and recombinant human KLK2. Briefly, KLK2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to KAL-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-KLK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. When recombinant human KAL is immobilized at 2 ug/mL (100 uL/well), the concentration of KLK2 that produces 50% optimal binding response is found to be approximately 0.49 ug/mL.
• Molecular Mass: 50.1kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 80%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SERPINA4 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 [ Homo sapiens ]
• Official Symbol: SERPINA4
• Synonyms: KAL; KST; PI4; KLST; PI-4; kallistatin
• Gene ID: 5267
• mRNA Refseq: NM_006215.2
• Protein Refseq: NP_006206.2
• MIM: 147935
• UniProt ID: P29622