Active Recombinant Human SFRP5 Protein, HA-tagged

• Cat.No.: SFRP5-2621H
• Product Overview: Recombinant Human SFRP5 Protein (Glu30-His317) with an N-terminal HA (YPYDVPDYA) tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
• Source: CHO
• Species: Human
• Tag: HA
• Predicted N Terminal: Tyr (HA), Trp37 & Ser46
• Bio-activity: Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 μg/mL in the presence of 100 ng/mL Recombinant Mouse Wnt‑3a.
• Molecular Mass: 30-40 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: SFRP5 secreted frizzled related protein 5 [ Homo sapiens (human) ]
• Official Symbol: SFRP5
• Synonyms: SFRP5; secreted frizzled related protein 5; SARP3; secreted frizzled-related protein 5; FRP-1b; SARP-3; frizzled-related protein 1b; sFRP-5; secreted apoptosis related protein 3
• Gene ID: 6425
• mRNA Refseq: NM_003015
• Protein Refseq: NP_003006
• MIM: 604158
• UniProt ID: Q5T4F7