Active Recombinant Human SPAM1 protein, His-tagged

• Cat.No.: SPAM1-738H
• Product Overview: Recombinant Human SPAM1 protein(Leu 36 - Tyr 482), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu 36 - Tyr 482
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5, 10% trehalose.
• Bio-activity: The activity of Human PH20, His Tag is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >60,000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ]
• Official Symbol: SPAM1
• Synonyms: SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); hyaluronidase PH-20; HYAL5; PH 20; SPAG15; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; HYA1; PH20; HYAL1; HYAL3; PH-20; MGC26532
• Gene ID: 6677
• mRNA Refseq: NM_001174044
• Protein Refseq: NP_001167515
• MIM: 600930
• UniProt ID: P38567