Active Recombinant Human SPINT2 Protein, His-tagged

• Cat.No.: SPINT2-965H
• Product Overview: Recombinant Human SPINT2 (Accession # O43291) Met1-Lys197, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Met1-Lys197
• Predicted N Terminal: Ala28
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and CaCl2.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 value is <4.6 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Human SPINT2, His-tagged has a calculated MW of 21 kDa. In SDS-PAGE migrates as 27 kDa and 32 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SPINT2 serine peptidase inhibitor, Kunitz type, 2 [ Homo sapiens ]
• Official Symbol: SPINT2
• Synonyms: SPINT2; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2; kunitz-type protease inhibitor 2; HAI 2; Kop; placental bikunin; hepatocyte growth factor activator inhibitor type 2; PB; HAI2; DIAR3; HAI-2; FLJ45571
• Gene ID: 10653
• mRNA Refseq: NM_001166103
• Protein Refseq: NP_001159575
• MIM: 605124
• UniProt ID: O43291
• Chromosome Location: 19q13.2