Active Recombinant Human Spondin 1, Extracellular Matrix Protein, His-tagged

• Cat.No.: SPON1-408H
• Product Overview: Recombinant human SPON1protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal6-His tag.Phe29-Cys807.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 29-807 a.a.
• Description: Human SPON1 issynthesized as an 807 amino acid (aa) precursor that contains a 28 aa signal sequenceand a 779 aa mature region. The mature region includes an N-terminal reelinlike domain (aa 1-200), a centrally placed SPON1 type segment (aa 201-440), andsix C-terminal class 2 thrombospondin type I repeats.
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: Phe29
• Molecular Weight: 88.9 kDa
• Activity: Measured by itsability to enhance neurite outgrowth of dissociated E13 chick embryonicdorsal root ganglia (DRG) neurons. Able to significantly enhance neuriteoutgrowth when immobilized as a 3 μL droplet containing 100 ng on a nitrocellulosecoatedmicroplate.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 100μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions afterreconstitution.

Gene Information

• Gene Name: SPON1 spondin 1, extracellularmatrix protein [ Homo sapiens ]
• Official Symbol: SPON1
• Synonyms: SPON1; spondin1, extracellular matrix protein; KIAA0762; MGC10724; f-spondin; spondin-1;VSGP/F-spondin; OTTHUMP00000235296; spondin 1, (f-spondin) extracellularmatrix protein; vascular smooth muscle cell growth-promoting factor; VSGP
• Gene ID: 10418
• mRNA Refseq: NM_006108
• Protein Refseq: NP_006099
• MIM: 604989
• UniProt ID: Q9HCB6
• Chromosome Location: 11p15.2
• Function: protein binding