Active Recombinant Human SRSF1, His-tagged

• Cat.No.: SRSF1-8494H
• Product Overview: Recombinant Human SRSF1, fused with His tag, was expressed in Sf21 Cells.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Description: ASF also known as SF2 is a member of the SR protein family. It is an essential pre-mRNA splicing factor required for both single and alternative splicing. Phosphorylation on serine residues located within the SR domain directly regulates ASF/SF2 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, such as U1-70K, U2AF and other SR proteins, ASF/SF2 also directly or indirectly interacts with HIV regulatory protein Rev, the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, and numerous transcription factors, thereby suggesting a potential role of ASF/SF2 in coordinating transcription and pre-mRNA splicing .
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 1-5 units are sufficient for a gel mobility shift assay in a 20 μl reaction, 100 units are sufficient for a protein-protein interaction assay and 100-500 units are required for a splicing assay in a 20 μl reaction.
• AA Sequence: SGGGVIRGPA GNNDCRIYVG NLPPDIRTKD IEDVFYKYGA IRDIDLKNRR GGPPFAFVEF EDPRDAEDAV YGRDGYDYDG YRLRVEFPRS GRGTGRGGGG GGGGGAPRGR YGPPSRRSEN RVVVSGLPPS GSWQDLKDHM REAGDVCYAD VYRDGTGVVE FVRKEDMTYA VRKLDNTKFR SHEGETAYIR VKVDGPRSPS YGRSRSRSRS RSRSRSRSNS RSRSYSPRRS RGSPRYSPRH SRSRSRT
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 350 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: SRSF1 serine/arginine-rich splicing factor 1 [ Homo sapiens ]
• Official Symbol: SRSF1
• Synonyms: SRSF1; serine/arginine-rich splicing factor 1; SFRS1, splicing factor, arginine/serine rich 1; alternate splicing factor; ASF; MGC5228; pre mRNA splicing factor SF2; P33 subunit; SF2; SF2p33; splicing factor 2; SR splicing factor 1; SRp30a; ASF-1; alternative-splicing factor 1; splicing factor, arginine/serine-rich 1; pre-mRNA-splicing factor SF2, P33 subunit; SFRS1; FLJ53078
• Gene ID: 6426
• mRNA Refseq: NM_006924
• Protein Refseq: NP_008855
• MIM: 600812
• UniProt ID: Q07955
• Chromosome Location: 17q22
• Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem
• Function: RNA binding; RS domain binding; nucleotide binding; protein binding