Active Recombinant Human TFRC protein, His-Avi-tagged, Biotinylated

• Cat.No.: TFRC-2029HB
• Product Overview: Biotinylated Recombinant Human TFRC protein(P02786)(Cys89-Phe760), fused with His tag and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-Transferrin R Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 41.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 80-85 kDa based on Tris-Bis PAGE result.
• Protein Length: Cys89-Phe760
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: TFRC transferrin receptor (p90, CD71) [ Homo sapiens ]
• Official Symbol: TFRC
• Synonyms: TFRC; transferrin receptor (p90, CD71); transferrin receptor protein 1; CD71; TFR1; T9; TR; TFR; p90; TRFR
• Gene ID: 7037
• mRNA Refseq: NM_001128148
• Protein Refseq: NP_001121620
• MIM: 190010
• UniProt ID: P02786