Active Recombinant Human TGFBI Protein, His-tagged

• Cat.No.: TGFBI-211H
• Product Overview: Recombinant Human TGFBI (Gly24-His683), with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rh beta IG-H3, immobilized at 5 μg/mL (100 μL/well), can induce greater than 45% cell adhesion.
• Molecular Mass: Predicted Molecular Mass: 73 kDa
  SDS-PAGE: 65-70 kDa, reducing conditions
• Protein length: Gly24-His683
• N-terminal Sequence Analysis: Gly24
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: TGFBI transforming growth factor beta induced [ Homo sapiens (human) ]
• Official Symbol: TGFBI
• Synonyms: TGFBI; transforming growth factor beta induced; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1; transforming growth factor-beta-induced protein ig-h3; RGD-CAP; RGD-containing collagen-associated protein; beta ig-h3; betaig-h3; kerato-epithelin; transforming growth factor beta-induced 68kDa; transforming growth factor, beta-induced, 68kD
• Gene ID: 7045
• mRNA Refseq: NM_000358
• Protein Refseq: NP_000349
• MIM: 601692
• UniProt ID: Q15582