| Source : |
Yeast |
| Species : |
Human |
| Tag : |
His |
| Form : |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Bio-activity : |
Recombinant Human TMPRSS2 His tag protein enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Boc-Gln-Ala-Arg-AMC), The Km is 21.93μM. Measured by Camostat Mesylate inhibit ratio on TMPRSS2, which can cleave fluorogenic peptide substrate (Boc-Gln-Ala-Arg-AMC). The Camostat Mesylate inhibit EC50 is 0.03347-0.07945μM. |
| Molecular Mass : |
44.8 kDa |
| Protein length : |
106-492aa |
| AA Sequence : |
WKFMGSKCSNSGIECDSSGTCINPS NWCDGVSHCPGGEDENRCVRLYGPN FILQVYSSQRKSWHPVCQDDWNENY GRAACRDMGYKNNFYSSQGIVDDSG STSFMKLNTSAGNVDIYKKLYHSDA CSSKAVVSLRCIACGVNLNSSRQSR IVGGESALPGAWPWQVSLHVQNVHV CGGSIITPEWIVTAAHCVEKPLNNP WHWTAFAGILRQSFMFYGAGYQVEK VISHPNYDSKTKNNDIALMKLQKPL TFNDLVKPVCLPNPGMMLQPEQLCW ISGWGATEEKGKTSEVLNAAKVLLI ETQRCNSRYVYDNLITPAMICAGFL QGNVDSCQGDSGGPLVTSKNNIWWL IGDTSWGSGCAKAYRPGVYGNVMVF TDWIYRQMRADG |
| Purity : |
Greater than 85% as determined by SDS-PAGE. |
| Storage : |
Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. |
