Active Recombinant Human TNFSF9 Protein (Leu 24 - Gln 186), His-tagged

• Cat.No.: TNFSF9-231H
• Product Overview: Recombinant Human TNFSF9 Protein is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1). Predicted N-terminus: Leu 24. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu 24 - Gln 186
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Human TNFSF9 Ligand, Fc-tag at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFSF9 protein, His-tagged with a linear range of 0.5-16 ng/mL (QC tested).
• Molecular Mass: 19.2 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF9 tumor necrosis factor (ligand) superfamily, member 9 [ Homo sapiens ]
• Official Symbol: TNFSF9
• Synonyms: TNFSF9; 4 1BB L; 4-1BBL; 4-1BB ligand; CD137L; 4-1BB-L
• Gene ID: 8744
• mRNA Refseq: NM_003811
• Protein Refseq: NP_003802
• MIM: 606182
• UniProt ID: P41273