Active Recombinant Human TPI1 Protein, His-tagged

• Cat.No.: TPI1-10H
• Product Overview: Recombinant Human TPI enzyme (active) with a N-terminal His-tag was produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Specification

• Description: This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.
• Source: Nicotiana benthamiana
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Active. >1000 mU/mg. Measuring the conversion of dihydroxyacetone phosphate (DHAP) to glyceradehyde-3-phosphate (GAP).
• Molecular Mass: 54 kDa dimer (2x 27 kDa subunits)
• Purity: >99%
• Applications: SDS-PAGE, Western Blot, functional assays
• Storage: Lyophilized materials are stable for up to twelve months from date of receipt at -70 centigrade.
  It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: 20 mM Tris-HCl, pH 7.5, 175 mM Trehalose Dihydrate and 0.9 mM DTT.

Gene Information

• Gene Name: TPI1 triosephosphate isomerase 1 [ Homo sapiens (human) ]
• Official Symbol: TPI1
• Synonyms: TPI1; triosephosphate isomerase 1; TIM; TPI; TPID; HEL-S-49; triosephosphate isomerase; epididymis secretory protein Li 49; methylglyoxal synthase; triose-phosphate isomerase; EC 4.2.3.3; EC 5.3.1.1
• Gene ID: 7167
• mRNA Refseq: NM_000365
• Protein Refseq: NP_000356
• MIM: 190450
• UniProt ID: P60174