Active Recombinant Human UBE2B Protein, His-tagged

• Cat.No.: UBE2B-531H
• Product Overview: Recombinant Human UBE2B (Accession # NP_003328), fused with an N-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: X mg/ml (X µM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
• Bio-activity: Recombinant Human His6-HR6B/UBE2B is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-HR6B/UBE2B concentration of 0.1-1 µM.
• Molecular Mass: Recombinant Human UBE2B, His-tagged has a calculated MW of 19 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: UBE2B ubiquitin-conjugating enzyme E2B [ Homo sapiens ]
• Official Symbol: UBE2B
• Synonyms: UBE2B; ubiquitin-conjugating enzyme E2B; ubiquitin conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2 B; HHR6B; RAD6B; UBC2; E2 protein; RAD6 homolog B; ubiquitin-protein ligase B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); HR6B; E2-17kDa
• Gene ID: 7320
• mRNA Refseq: NM_003337
• Protein Refseq: NP_003328
• MIM: 179095
• UniProt ID: P63146
• Chromosome Location: 5q31.1