Active Recombinant Human VEGFC protein, His-SUMO-tagged

Cat.No. : VEGFC-3153H
Product Overview : Active Recombinant Human VEGFC protein(P49767)(47~413 aa), fused with N-terminal His tag and SUMO tag, was expressed in E.coli.
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Species : Human
Source : E.coli
Tag : His&SUMO
Protein Length : 47-413 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339µg/mL.
Molecular Mass : 55.3kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
Gene Name VEGFC vascular endothelial growth factor C [ Homo sapiens ]
Official Symbol VEGFC
Synonyms VEGFC; vascular endothelial growth factor C; VRP; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein; Flt4-L;
Gene ID 7424
mRNA Refseq NM_005429
Protein Refseq NP_005420
MIM 601528
UniProt ID P49767

Not For Human Consumption!

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