Active Recombinant Human XIAP protein, Avi-tagged

• Cat.No.: XIAP-3103H
• Product Overview: Active Recombinant Human XIAP protein(NP_001158.2) (Leu 121-Thr 356) was expressed in E. coli, fused with an AVI tag at the C-terminus, and additonal two amino acids (Gly & Pro) at the N-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: Avi
• Protein Length: 121-356 aa
• Form: Lyophilized from sterile 25mM Tris, 10mM DTT, 1% glycerol, 0.2M Glutamine Potassium, pH 8.0.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI with a linear range of 0.125-1.0 μg/ml.
• Molecular Mass: The recombinant human XIAP comprises 253 amino acids and has a predicted molecular mass of 29.1 kDa as estimated in SDS-PAGE under non-reduced conditions.
• Purity: > 75 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: XIAP X-linked inhibitor of apoptosis [ Homo sapiens ]
• Official Symbol: XIAP
• Synonyms: XIAP; X-linked inhibitor of apoptosis; API3, baculoviral IAP repeat containing 4 , BIRC4; baculoviral IAP repeat-containing protein 4; hILP; X-linked IAP; IAP-like protein; E3 ubiquitin-protein ligase XIAP; inhibitor of apoptosis protein 3; baculoviral IAP repeat-containing 4; X-linked inhibitor of apoptosis protein; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3; FLJ26913
• Gene ID: 331
• mRNA Refseq: NM_001167
• Protein Refseq: NP_001158
• MIM: 300079
• UniProt ID: P98170