Active Recombinant IAV H3N2 Neuraminidase Protein (Ala46-Ile469), C-6×His tagged

• Cat.No.: NA-10
• Product Overview: Recombinant IAV H3N2 Neuraminidase Protein, Ala46-Ile469, vasodilator-stimulated phosphoprotein tetramerization domain with a C-terminal 6-His tag was expressed in Spodoptera frugiperda, Sf 21 (Insect cells).

Specification

• Description: Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moieties on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
• Source: Insect cells
• Species: IAV
• Tag: C-6×His
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. The specific activity is > 10,000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 53 kDa
  SDS-PAGE: 57-65 kDa, reducing conditions
• Protein Length: Ala46-Ile469
• N-terminal Sequence Analysis: Ser (of tetramerization domain)
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2, DTT and Glycerol.
• Shipping: The product is shipped with dry ice or equivalent.
• Official Symbol: NA
• Synonyms: Neuraminidase; NA; EC:3.2.1.18; NANH; Sialidase; Viral Neuraminidase
• UniProt ID: Q91MA2

Gene Information

• Official Symbol: NA
• Synonyms: Neuraminidase; NA; EC:3.2.1.18; NANH; Sialidase; Viral Neuraminidase
• UniProt ID: Q91MA2