Active Recombinant Influenza A H1N1 (A/USSR/90/1977) NA protein(Met1-Lys470)

• Cat.No.: NA-3400H
• Product Overview: Recombinant Influenza virus A (A/USSR/90/1977)(H1N1)) Neuraminidase (P03469.2) (Met1-Lys470), termed as NA, was expressed in HEK293.

Specification

• Source: HEK293
• Species: H1N1
• Tag: N/A
• Protein length: Met1-Lys470
• Form: Lyophilized from sterile PBS, 1%Triton X-100 PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. The specific activity is > 150 U. The specific activity is > 1500 U.
• Molecular Mass: The recombinant Influenza virus A (A/USSR/90/1977)(H1N1)) Neuraminidase consists of 470 amino acids and predicts a molecular mass of 51.9 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.