Species : |
Influenza A H7N7 (A/chicken/Netherlands/1/03) |
Source : |
Insect Cells |
Tag : |
His |
Protein Length : |
Met8-Phe544 |
Form : |
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
1. Measured in hemagglutination test using 1% chicken erythrocyte. The HA titer is 0.1-0.5 μg/ml for 1% CRBC.2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. |
Molecular Mass : |
The secreted recombinant hemagglutinin of Influenza A virus (A/chicken/Netherlands/1/03 (H7N7)) comprises 530 amino acids and has a calculated molecular mass of 59.4 kDa as estimated in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 80 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |