Active Recombinant Influenza B (B/Malaysia/2506/2004) HA Protein, His-tagged

• Cat.No.: HA-403V
• Product Overview: Active Recombinant Influenza B (B/Malaysia/2506/2004) HA Protein(ACO05957.1)(Met 1-Ala 556)(HA1+HA2,uncleaved), was expressed in Insect Cells, fused with a C-terminal polyhistidine tag.

Specification

• Species: Influenza B
• Source: Insect Cells
• Tag: His
• Protein Length: 1-556 aa
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.005-0.02μg/mL for 1% GRBC.
• Molecular Mass: The recombinant influenza B virus hemagglutinin (B/Malaysia/2506/2004) HA consists 552 amino acids and predicts a molecular mass of 60 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.