Active Recombinant Monkeypox virus(strain Zaire-96-I-16) H3L protein, His-tagged

• Cat.No.: H3L-001M
• Product Overview: Recombinant Monkeypox virus(strain Zaire-96-I-16) H3L protein(Met 1 - Phe 282), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Monkeypox virus(strain Zaire-96-I-16)
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag at 1 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV H3L Antibody with a linear range of 4-63 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 34.6 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Met1-Phe282
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.