Active Recombinant Monkeypox virus(strain Zaire-96-I-16) H3L protein, His-tagged
Cat.No. : | H3L-001M |
Product Overview : | Recombinant Monkeypox virus(strain Zaire-96-I-16) H3L protein(Met 1 - Phe 282), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Monkeypox virus(strain Zaire-96-I-16) |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag at 1 μg/mL (100 μL/well) can bind Anti-Monkeypox virus/MPXV H3L Antibody with a linear range of 4-63 ng/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 34.6 kDa. The protein migrates as 35-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Met1-Phe282 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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