Active Recombinant Mouse Bone Morphogenetic Protein 10

• Cat.No.: Bmp10-575M
• Product Overview: Recombinant Mouse Bmp10protein was expressed in Chinese Hamster Ovary cell line. Asn314-Arg421.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• Protein Length: 314-421 a.a.
• Description: Bmp10, along with BMP9,GDF5, 6, and 7, belongs to a subgroup of TGFβ superfamily proteins thatsignal through heterodimeric complexes composed of type I and type II BMPreceptors. Proteolytic removal of the propeptide from the 60 kDa proproteinyields a 12 kDa mature Bmp10 which forms disulfidelinked nonglycosylatedhomodimers.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Asn314
• Molecular Weight: 12.1 kDa (monomer)
• Activity: Measured by itsability to induce alkaline phosphatase production by MC3T3-E1 mouseosteoblastic cells. The ED50 for this effect is typically 15-75 ng/mL.
• Endotoxin Level: < 1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 10 kDa,reducing conditions.
• Purity: > 95%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: 250 μg/mL in 4 mMHCl containing at least 0.1% human or bovine serum albumin.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Bmp10 bone morphogeneticprotein 10 [ Mus musculus ]
• Official Symbol: Bmp10
• Synonyms: Bmp10; bonemorphogenetic protein 10; BMP-10
• Gene ID: 12154
• mRNA Refseq: NM_009756
• Protein Refseq: NP_033886
• Chromosome Location: 6; 6 D2
• Function: cytokine activity;growth factor activity; receptor serine/threonine kinase binding