Active Recombinant Mouse CD244 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD244-2849M
• Product Overview: Biotinylated Recombinant Mouse CD244 protein(Val 24-Leu 211), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Val 24-Leu 211
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Mouse 4-1BB / TNFRSF9 (24-211), Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse 4-1BB Ligand, Fc Tag with a linear range of 0.3-2 ng/mL.Immobilized Mouse 4-1BB Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse 4-1BB / TNFRSF9 (24-211), Avitag,His Tag with a linear range of 0.3-20 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.The protein has a calculated MW of 24.5 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: Cd244 CD244 natural killer cell receptor 2B4 [ Mus musculus ]
• Official Symbol: CD244
• Synonyms: CD244; CD244 natural killer cell receptor 2B4; natural killer cell receptor 2B4; NK cell receptor 2B4; NK cell type I receptor protein 2B4; non MHC restricted killing associated; non-MHC restricted killing associated; 2B4; C9.1; Ly90; NAIL; Nmrk; NKR2B4; SLAMF4; F730046O15Rik
• Gene ID: 18106
• mRNA Refseq: NM_018729
• Protein Refseq: NP_061199