Active Recombinant mouse CD300C Protein, mIgG2A-tagged

• Cat.No.: CD300C-3087M
• Product Overview: Recombinant mouse CD300C (Cys22-Ser193) protein with Fc fragment of a Mouse IgG2A (Glu98-Lys330) at C-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Mouse
• Source: CHO
• Tag: Fc
• Protein Length: 22-193;98-330 a.a.
• Predicted N Terminal: Cys22
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and serially diluted Recombinant Mouse CD300c/LMIR2 Fc Chimera. The ED50 for this effect is 0.6‑3 µg/mL.
• Molecular Mass: 46 kDa (monomer)
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: Cd300c2 CD300C molecule 2 [ Mus musculus (house mouse) ]
• Official Symbol: Cd300c2
• Synonyms: Cd300c2; CD300C molecule 2; CMRF-35-like molecule 4; CLM; CMRF35-like molecule; Cd300C antigen; Cd300D antigen; MAIR-2; dendritic cell-derived Ig-like receptor 1; dendritic cell-derived immunoglobulin receptor 1; immunoglobulin superfamily, member 7; leukocyte mono-Ig-like receptor 2; myeloid-associated immunoglobulin-like receptor 2; myeloid-associated immunoglobulin-like receptor II; CLM4; CLM-4; DIgR1; Igsf7; Lmir2; Cd300c; Cd300d; MAIR-II
• Gene ID: 140497
• mRNA Refseq: NM_134158
• Protein Refseq: NP_598919
• UniProt ID: Q7TSN2