Active Recombinant Mouse Cell Adhesion Molecule 1, His-tagged

• Cat.No.: Cadm1-5604M
• Product Overview: Recombinant Mouse Cadm1protein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Ile42-Asp376.

Specification

• Cat. No.: Cadm1-5604M
• Description: Cadm1 is a 75 kDaimmunoglobulin superfamily protein that mediates calcium-independent adhesiveinteractions, either homophilic or heterophilic with CRTAM (classI-restricted T cell-associated molecule), Necl1 (SynCAM3) or Nectin3.Alternate names for Cadm1 reflect its activities. As Cadm1, it isdownregulated in onsmall cell lung cancer. This may allow evasion ofantitumor responses, since Cadm1 interaction with CRTAM on activated NK andCD8+ T cells stimulates cytotoxicity and upregulates IFN-γ production.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ile42
• Molecular Weight: 38.8 kDa
• Activity: Measuredby its ability to inhibit rhBMP-4-induced alkaline phosphatase production byATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem.Biophys. Res. Commun. 315:272. The ED50 for this effect istypically 0.5-2 μg/mL in the presence of 75 ng/mL of rhBMP-4.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 76-87kDa, reducing conditions
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Cadm1 cell adhesion molecule 1[ Mus musculus ]
• Official Symbol: Cadm1
• Synonyms: Cadm1; celladhesion molecule 1; Bl2; ST17; Igsf4; Necl2; RA175; Tslc1; Igsf4a; RA175A;RA175B; RA175C; RA175N; SgIGSF; SynCam; AI987920; 2900073G06Rik;3100001I08Rik; cell adhesion molecule 1; NECL-2; TSLC-1; nectin-like 2;nectin-like protein 2; immunosuperfamily protein Bl2; synaptic cell adhesionmolecule; tumor suppressor in lung cancer 1; immunoglobulin superfamilymember 4; immunoglobulin superfamily member 4A; spermatogenic immunoglobulinsuperfamily; SynCam1
• Gene ID: 54725
• mRNA Refseq: NM_018770
• Protein Refseq: NP_061240
• UniProt ID: Q8R5M8
• Chromosome Location: 9; 9 B-C
• Pathway: Adherens junctionsinteractions; Cell adhesion molecules (CAMs); Cell junction organization;Cell-Cell communication; Nectin/Necl trans heterodimerization
• Function: PDZ domain binding;cell adhesion molecule binding; protein C-terminus binding; protein binding;protein homodimerization activity; receptor binding