Active Recombinant Mouse Contactin 6, His-tagged

Species :	Mouse
Source :	CHO
Tag :	His
Protein Length :	20-998 a.a.
Description :	Cntn6, also known asNB3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPIlinked neuronaladhesion proteins. Mature mouse Cntn6 consists of six immunoglobulinlike domains,a flexible linker region, four fibronectin type III domains, and a GPI anchor.Mouse Cntn6 shares 90% and 96% amino acid (aa) sequence identity with human andrat Cntn6, respectively. Alternate splicing generates an isoform with a 17 aadeletion in the first Iglike domain.
Form :	Lyophilized from a 0.2μm filtered solution in PBS.
N-terminalSequence :	Glu20
Molecular Weight :	109.2 kDa
Activity :	Measuredby the ability of the immobilized protein to support the adhesion of Neuro2a mouseneuroblastoma cells (ATCC: CCL131). When 5 x 104 cells/well are addedto rmCntn6 coated plates (10 μg/mL, 100 μL/well), approximately 40-60% will adhereafter 30 minutes at 37 °C. Optimal dilutions should be determined by each laboratoryfor each application.
Endotoxin Level :	<1.0 EUper 1 μg of the protein by the LAL method.
Purity :	>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :	Reconstituteat 100 μg/mL in sterile PBS
Storage :	Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.

Gene Name	Cntn6 contactin6 [ Mus musculus ]
Official Symbol	Cntn6
Synonyms	Cntn6; contactin6; NB-3; mNB-3; neural recognition molecule NB-3
Gene ID	53870
mRNA Refseq	NM_017383
Protein Refseq	NP_059079
UniProt ID	Q9JMB8
Chromosome Location	6; 6 E2