Dpp7-416M
Optional Service: Optional requirements on this protein
Product Overview :
Recombinantmouse Dpp7 protein was expressed in Murine myeloma cell line, NS0-derived, with a C-terminal10-His tag.Asp34-Arg506
Description :
The protein encodedby this gene is a post-proline cleaving aminopeptidase expressed in quiescentlymphocytes. The resting lymphocytes are maintained through suppression ofapoptosis, a state which is disrupted by inhibition of this novel serineprotease. The enzyme has strong sequence homology with prolylcarboxypeptidaseand is active at both acidic and neutral pH.
Source :
Mammalian cells
Form :
Lyophilized from a0.2 μm filtered solution in Tris and Nacl.
N-terminal Sequence :
Asp34
Molecular Weight :
54 kDa
Activity :
Measured by itsability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin(GP-AMC). The specific activity is > 2,500 pmoles/min/μg, as measuredunder the described conditions. See Activity Assay Protocol.
Endotoxin Level :
<1.0 EU per 1 μgof the protein by the LAL method.
Purity :
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :
Reconstitute at 200μg/mL in 25 mM Tris and 150 mM NaCl, pH 7.5.
Storage :
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3months, -20 to -70 °C under sterile conditions after reconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.