Active Recombinant Mouse Ectonucleotide Pyrophosphatase/Phosphodiesterase 7, His-tagged

• Cat.No.: Enpp7-452M
• Product Overview: RecombinantMouse Enpp7protein was expressed in Murine myeloma cell line. Ala22-Gln421,with a C terminal 10 His tag.

Specification

• Cat. No.: Enpp7-452M
• Description: Enpp7is expressedin the intestines and in bile. It shares 30 36% homology with the members ofthe nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharingno similarities with neutral or acid SMase. Its main function is thedigestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramideand phosphorylcholine. Enpp7 is reported tohydrolyse and inactivate plateletactivating factor (PAF) by a phospholipase Ctypeactivity. Studies show a decrease in Enpp7 activityin human colorectal adenocarcinomas and human colorectal carcinomas, whichindicate a potential role of Enpp7 in human coloncancer.
• Source: Mammalian cells
• Form: Suppliedas a 0.2 um filtered solution in Tris and NaCl.
• N-terminalSequence: Ala22
• MolecularWeight: 47 kDa
• Activity: Measuredby its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine.The phosphorylcholine is cleaved by rhAlkaline Phosphatase/ALPL and thephosphate is detected or measured by a Malachite Green Phosphate DetectionKit. The specific activity is > 13000 pmoles/min/μg, as measured under thedescribed conditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
• Purity: >95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, 20 to 70°C as supplied. 3 months, 20 to 70°C understerile conditions after opening.

Gene Information

• Gene Name: Enpp7 ectonucleotidepyrophosphatase/phosphodiesterase 7 [ Mus musculus ]
• Official Symbol: Enpp7
• Synonyms: Enpp7;ectonucleotide pyrophosphatase/phosphodiesterase 7; Gm254; Alk-SMase; ectonucleotidepyrophosphatase/phosphodiesterase family member 7; EC 3.1.4.12
• Gene ID: 238011
• mRNA Refseq: NM_001030291
• Protein Refseq: NP_001025462
• UniProt ID: Q3TIW9
• Chromosome Location: 11
• Pathway: Metabolic pathways;Sphingolipid metabolism
• Function: NOT nucleotidaseactivity; sphingomyelin phosphodiesterase activity