Active Recombinant Mouse Glial Cell Line Derived Neurotrophic Factor Family Receptor Alpha 3, His-tagged and Fc-tagged

• Cat.No.: Gfra3-5619M
• Product Overview: Recombinant Mouse Gfra3protein was expressed in Sf 21 with the Fc part of human IgG1 and aC-terminal 6-His tag. Glu34-Arg379.

Specification

• Species: Mouse
• Source: S.frugiperda
• Tag: Fc&His
• Protein Length: 34-379 a.a.
• Description: Mouse Gfra3 is amember of the GDNF family of receptors. It is one of four known members, allof which contain three conserved cysteine repeats. It is synthesized as a 397aa precursor, with a hydrophobic signal sequence of 28 aa, a 343 aa maturesegment, and a propeptide segment of 26 aa that is removed in the matureprotein.
• Form: Lyophilized from a0.2 μm filtered solution in PBS and NaCl.
• N-terminalSequence: Met
• Molecular Weight: 66.2 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized Recombinant Mouse(rm) Artemin at 1 μg/mL can bind rmGfra3 Fc Chimera with an apparent KD <5 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 70-75kDa, reducing conditions
• Purity: >80%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Gfra3 glial cell line derivedneurotrophic factor family receptor alpha 3 [ Mus musculus ]
• Official Symbol: Gfra3
• Synonyms: Gfra3;glial cell line derived neurotrophic factor family receptor alpha 3; Y15110;GFRalpha3; GDNF family receptor alpha-3; GFR alpha-3; GFR-alpha-3;GDNFR-alpha-3; GDNF receptor alpha-3
• Gene ID: 14587
• mRNA Refseq: NM_010280
• Protein Refseq: NP_034410
• UniProt ID: O35118
• Chromosome Location: 18 B1; 18
• Function: axon guidancereceptor activity; coreceptor activity; protein binding; receptor activity