Active Recombinant Mouse Gpc1 protein, His-tagged

• Cat.No.: Gpc1-153M
• Product Overview: Recombinant Mouse Gpc1(Asp24-Ser529) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: Asp24-Ser529
• Predicted N Terminal: Asp24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 1 at 3 µg/mL (100 µL/well) will bind rhFGF-basic with an apparent KD < 1 nM.
• Molecular Mass: Predicted Molecular Mass: 56.4 kDa;SDS-PAGE: 57-62 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: Gpc1 glypican 1 [ Mus musculus ]
• Official Symbol: Gpc1
• Synonyms: GPC1; glypican 1; glypican-1; AI462976
• Gene ID: 14733
• mRNA Refseq: NM_016696
• Protein Refseq: NP_057905
• UniProt ID: Q9QZF2
• Chromosome Location: 1; 1 D
• Pathway: Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Inactivation of Cdc42 and Rac, organism-specific biosystem; Role of Abl in Robo-Slit signaling, organism-specific biosystem; Signaling by Robo receptor, organism-specific biosystem
• Function: collagen V binding; heparan sulfate proteoglycan binding