Active Recombinant Mouse Gremlin 1, His-tagged

• Cat.No.: Grem1-5635M
• Product Overview: RecombinantMouse Grem1 protein was expressed in Murine myeloma cellline. Lys25-Asp184,with a C-terminal 10-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 25-184 a.a.
• Description: Grem1 wasidentified in a Xenopus expression-cloning screen as a dorsalizing factorthat can induce a secondary axis. A rat homolog, called Grem1, was identifiedas a cDNA that was downregulated in v-mos transfected cells. Grem1 belongs tothe DAN family of secreted glycoproteins that are BMP antagonists. Othermembers of the family include: cerberus, Dante, PRDC, caronte and DAN. DAN familymembers share a cysteine-rich domain that is structurally related to thecysteine-knot motif found in TGF-β superfamily ligands. In vitro, Grem1 bindsBMP-4 and BMP-2 indicating that it might interfere with BMP signaling.
• Form: Lyophilized from a0.2 um filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminalSequence: Lys25
• MolecularWeight: 19.6 kDa
• Activity: Measuredby its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5mouse chondrogenic cells. The ED50 for this effect is typically 0.4-2 μg/mLin the presence of 75 ng/mL of rhBMP-4.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 23-31kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 250 μg/mLin sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 monthsfrom date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C understerile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.

Gene Information

• Gene Name: Grem1 gremlin 1 [ Musmusculus ]
• Official Symbol: Grem1
• Synonyms: Grem1; gremlin 1;ld; Drm; Cktsf1b1; gremlin-1; down-regulated in v-mos-transformed cells;cysteine knot superfamily 1, BMP antagonist 1; down-regulated inMos-transformed cells protein
• Gene ID: 23892
• mRNA Refseq: NM_011824
• Protein Refseq: NP_035954
• UniProt ID: O70326
• Chromosome Location: 2
• Function: cytokine activity