Active Recombinant Mouse HIPK2, GST-tagged

• Cat.No.: HIPK2-1414M
• Product Overview: Recombinant mouse HIPK2 (153-564) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Mouse
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 153-564 aa
• Description: HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors. HIPK2 enhances expression of p53 target genes that resulting in growth arrest and the enhancement of UV-induced apoptosis that could be inhibited by antisense to HIPK2. HIPK2 as a target for MDM2 mediated ubiquitin-dependent degradation that only occurred in growth-arresting conditions when MDM2 was efficiently induced by p53. The knockdown of HIPK2 using small interfering RNA reduced p53 binding and activation of p53R2 that resulting in impaired UV-induced DNA repair.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 110 nmol/min/mg
• Molecular Mass: ~72 kDa
• Purity: >95%
• Applications: Kinase Assay, Western Blot
• Storage: Store at–70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: Hipk2 homeodomain interacting protein kinase 2 [ Mus musculus ]
• Official Symbol: HIPK2
• Synonyms: HIPK2; homeodomain interacting protein kinase 2; homeodomain-interacting protein kinase 2; nuclear body-associated kinase 1; sialophorin tail-associated nuclear serine/threonine-protein kinase; Stank; 1110014O20Rik; B230339E18Rik
• Gene ID: 15258
• mRNA Refseq: NM_001136065
• Protein Refseq: NP_001129537
• Pathway: Wnt Signaling Pathway NetPath, organism-specific biosystem
• Function: ATP binding; NOT DNA binding; RNA polymerase II activating transcription factor binding; contributes_to RNA polymerase II transcription coactivator activity; SMAD binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity; transcription corepressor activity; transcription corepressor activity; transferase activity; transferase activity, transferring phosphorus-containing groups; virion binding