Active Recombinant Mouse IL12A & IL12B protein, hFc-tagged

• Cat.No.: IL12A & IL12B-2192M
• Product Overview: Recombinant Mouse IL12A & IL12B protein(P43431 & P43432)(Met1-Ala215 & Met1-Ser335), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ala215 & Met1-Ser335
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized Mouse IL12RB2 (His Tag) at 2 μg/mL (100 μL/well) can bind Mouse IL-12 (hFc Tag), the EC50 is 30-340 ng/mL. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1-0.8 μg/mL.
• Molecular Mass: The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.