Active Recombinant Mouse IL12A & IL12B protein, hFc-tagged
Cat.No. : | IL12A & IL12B-2192M |
Product Overview : | Recombinant Mouse IL12A & IL12B protein(P43431 & P43432)(Met1-Ala215 & Met1-Ser335), fused with hFc tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | hFc |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Immobilized Mouse IL12RB2 (His Tag) at 2 μg/mL (100 μL/well) can bind Mouse IL-12 (hFc Tag), the EC50 is 30-340 ng/mL. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.1-0.8 μg/mL. |
Molecular Mass : | The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Ala215 & Met1-Ser335 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 85 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionMass spectrometry identifies IL12A's post-translational modifications and their roles.
Knockout models validate IL12A's roles in immune response modulation.
Protein footprinting assays reveal IL12A's binding interfaces with receptors.
ELISA and stability assays quantify IL12A secretion and stability in culture.
SPR characterizes IL12A's interactions with antibodies and inhibitors.
Cellular assays gauge IL12A's cytokine induction in immune cells.
Isothermal titration calorimetry measures IL12A's binding affinity with receptors.
Structural biology techniques analyze IL12A genetic variant impacts.
Site-directed mutagenesis probes IL12A's functional domains and interactions.
Bioluminescence imaging tracks IL12A expression dynamics in vivo.
Customer Reviews (3)
Write a reviewSuitable for co-immunoprecipitation.
Consistent in bioactivity.
Good for receptor binding studies.
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