Active Recombinant Mouse Intercellular Adhesion Molecule 5, Telencephalin, Fc-tagged

• Cat.No.: Icam5-5641M
• Product Overview: RecombinantMouse Icam5 protein was expressed in Murine myeloma cell line with the Fcpart of Human IgG1 Pro100-Lys330 (C-terminus) and Mouse Icam5 Leu31-Arg828(Pro47Arg) (N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 31-828 a.a.
• Description: Icam5 isan integral membrane glycoprotein expressed in neurons of mammalian telencephalons.Icam5 is a member of the immunoglobulin superfamily and shares 38-55% aminoacid homology with other ICAMs. Structurally, Icam5 contains nine Ig domainsthat included 15 N-glycosylation sites, a single transmembrane region, and C-terminalcytoplasmic tail. As with other members of the ICAM family, Icam5 has beenshown to be involved in cellular adhesion.
• Form: Lyophilizedfrom a 0.2 um filtered solution in Tris-Citrate and NaCl.
• N-terminalSequence: Leu31
• MolecularWeight: 112 kDa (monomer)
• Activity: Measuredby the ability of the immobilized protein to support the adhesion ofPMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemiacells. When 5x104 cells/well are added to rh Icam5/FcChimera coated plates (25 μg/mL with 100 μL/well), > 25% cells will adhereafter 1 hour incubation at 37°C.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 166 kDa,reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 400 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Icam5 intercellular adhesionmolecule 5, telencephalin [ Mus musculus ]
• Official Symbol: Icam5
• Synonyms: Icam5; intercellularadhesion molecule 5, telencephalin; TLN;CD50; Tlcn; Icam3; ICAM-3; intercellular adhesion molecule 5; ICAM-5;telencephalin; intercellular adhesion molecule 3
• Gene ID: 15898
• mRNA Refseq: NM_008319
• Protein Refseq: NP_032345
• UniProt ID: Q60625
• Chromosome Location: 9
• Function: protein binding