Active Recombinant Mouse Nrp2 protein, Fc-tagged

• Cat.No.: NRP2-10899M
• Product Overview: Recombinant Mouse Nrp2(Gln23-Asp863) fused with Fc of Mouse IgG2A(Glu98-Lys330) at C-terminal was expressed in NS0.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 23-863 a.a.
• Predicted N Terminal: Gln23 predicted: no results obtained, sequencing might be blocked
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Neuropilin-2 Fc Chimera at 5 μg/mL (100 μL/well) can bind Recombinant Human VEGF165 in the presence of 2 μg/mL of heparin with an apparent Kd <1nM.
• Molecular Mass: Predicted Molecular Mass: 122 (monomer) kDa;
  SDS-PAGE: 125-135 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: Nrp2 neuropilin 2 [ Mus musculus ]
• Official Symbol: Nrp2
• Synonyms: NRP2; neuropilin 2; neuropilin-2; vascular endothelial cell growth factor 165 receptor 2; Np2; Np-2; Npn2; Npn-2; 1110048P06Rik
• Gene ID: 18187
• mRNA Refseq: NM_001077403
• Protein Refseq: NP_001070871
• UniProt ID: O35375
• Chromosome Location: 1; 1 C2
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; Neurophilin interactions with VEGF and VEGFR, organism-specific biosystem; NrCAM interactions, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by VEGF, organism-specific biosystem
• Function: heparin binding; metal ion binding; protein binding; receptor activity; semaphorin receptor activity