Active Recombinant Mouse Thop1 protein, His-tagged

• Cat.No.: Thop1-2555M
• Product Overview: Recombinant Mouse Thop1 protein(NP_073144.3)(Lys2-Cys687), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: Lys2-Cys687
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glyPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH or Mca-PLGPK(Dnp)-OH.The specific activity is > 100 pmoles/min/μg.
• Molecular Mass: The recombinant mouse THOP1 consists of 705 amino acids and has a calculated molecular mass of 80.1 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Thop1 thimet oligopeptidase 1 [ Mus musculus ]
• Official Symbol: Thop1
• Synonyms: THOP1; thimet oligopeptidase 1; thimet oligopeptidase; endopeptidase 24.15; EP24.15; AI131655; AI327041
• Gene ID: 50492
• mRNA Refseq: NM_022653
• Protein Refseq: NP_073144