Active Recombinant Mouse Tnfsf9, His-tagged

• Cat.No.: Tnfsf9-132M
• Product Overview: A DNA sequence encoding the extracellular domain (Arg 104-Glu 309) of mouse TNFSF9 precursor was expressed with a N-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: Human Cells
• Tag: His
• Protein Length: 104-309 a.a.
• Description: 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse 4-1BB Ligand at 20 μg/ml (100ul/well) can bind human 4-1BB with a linear range of 15.6-500 ng/ml .
• Molecular Mass: The secreted recombinant mouse TNFSF9 consists of 222 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70?
• Storage: Store it under sterile conditions at -20? to -80?. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Tnfsf9 tumor necrosis factor (ligand) superfamily, member 9 [ Mus musculus (house mouse) ]
• Official Symbol: Tnfsf9
• Synonyms: Tnfsf9; tumor necrosis factor (ligand) superfamily, member 9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817; tumor necrosis factor ligand superfamily member 9; 4-1BB ligand
• Gene ID: 21950
• mRNA Refseq: NM_009404
• Protein Refseq: NP_033430
• UniProt ID: P41274
• Chromosome Location: 17 D; 17
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding