Active Recombinant Mouse Txn1, His-tagged

• Cat.No.: Txn1-538M

Specification

• Species: Mouse
• Tag: His
• Description: Txn1 also known as thioredoxin 1, was an important protein in mouse that has many functions, such as enzyme binding, poly(A) RNA binding.
• Form: Lyophilized from 0.5% NH4HCO3.
• Bio-activity: Fully active with mammalian thioredoxin reductase. Activity can be measured with the thioredoxin-dependent reduction of insulin using rat recombinant thioredoxin reductase. The test mixture should contain 160 µM insulin and 0.2 mM NADPH in 0.1 M potassium phosphate, pH 7.0, containing 2 mM EDTA. In the presence of 5 µM mouse thioredoxin, 7 nM mammalian thioredoxin reductase will give an absorbance decrease of approximately 0.10/min at 340 nm.
• Notes: Make sure to reconstitute all of the lyophilized protein in the ampoule. After adding buffer, close the screw-cap again, shake vigorously, then centrifuge shortly, in order to recover liquid from tube cap and walls.
• Stability: 6 months
• Storage: Keep at 4 centigrade or alternatively reconstituted protein in aliquots at -20 centigrade. Lyophilized substance.
• Reconstitution: Reconstitute with H2O or buffer.

Gene Information

• Gene Name: Txn1 thioredoxin 1 [ Mus musculus ]
• Official Symbol: Txn1
• Synonyms: ADF; Txn; Trx1; AW550880; thioredoxin; ATL-derived factor; trx
• Gene ID: 22166
• mRNA Refseq: NM_011660
• Protein Refseq: NP_035790
• UniProt ID: P10639
• Chromosome Location: 4 B3; 4 31.87 cM
• Pathway: Cellular Senescence, organism-specific biosystem; Cellular responses to stress, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: enzyme binding; oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor; peptide disulfide oxidoreductase activity