| Description : |
Trypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester and amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl- L-arginine methyl ester (TAME), tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide (BAPNA), L-lysyl-p-nitroanilide, and benzoyl-L-tyrosine ethyl ester (BTEE). Serine protease inhibitors that inhibit recombinant trypsin include TLCK (N-p-tosyl-L-lysine chloromethyl ketone), PMSF (phenylmethanesulfonyl fluoride), benzamidine, soybean trypsin inhibitor, and ovomucoid. |
| Source : |
E. coli |
| Species : |
Porcine |
| Form : |
Sterile Filtered lyophilized powder. |
| Bio-activity : |
4500 USP units/mg protein. |
| AA Sequence : |
VGGYTCAANSIPYQVSLNSGSHFCG GSLINSQWVVSAAHCYKSRIQVRLG EHNIDVLEGNEQFINAAKIITHPNF NGNTLDNDIMLIKLSSPATLNSRVA TVSLPRSCAAAGTECLISGWGNTKS SGSSYPSLLQCLKAPVLSDSSCKSS YPGQITGNMICVGFLEGGKDSCQGD SGGPVVCNGQLQGIVSWGYGCAQKN KPGVYTKVCNYVNWIQQTIAAN |
| Unit Definition : |
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0 ml at pH7.6 and 25 centigrade, with BAEE as a substrate (1 cm light path). |
| Applications : |
Trypsin digestion: the suggested ratio is 1:50 to 1:1000 (w/w). |
| Notes : |
Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Stability : |
Recombinant Porcine Trypsin although stable at room temp for 1 week, should be stored desiccated below -18 centigrade.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Preservative : |
The Porcine Trypsin was lyophilized with mannitol as preservative. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized Porcine Trypsin in sterile 1mM HCl or 50mM HAC not less than 100 μg/mL, which can then be further diluted to other aqueous solutions. |
| Shipping : |
Shipped at Room temperature. |
| Official Symbol : |
Trypsin |
| Synonyms : |
Trypsin |
