Active Recombinant Rat Acvrl1 protein, His&hFc-tagged

• Cat.No.: Acvrl1-245R
• Product Overview: Recombinant Rat Acvrl1 protein(NP_071886.1)(Met1-Ala118), fused with His and hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Ala118
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953.The ED50 for this effect is typically 40-200 ng/mL in the presence of 2 ng/mL of recombinant human BMP9.
• Molecular Mass: The secreted recombinant rat ACVRL1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 343 amino acids with a predicted molecular mass of 38.7 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Acvrl1 activin A receptor type II-like 1 [ Rattus norvegicus ]
• Official Symbol: Acvrl1
• Synonyms: ACVRL1; activin A receptor type II-like 1; serine/threonine-protein kinase receptor R3; SKR3; TSR-I; Activin receptor likekinase 1; TGF-B superfamily receptor type I; R3; R-3; SETHKIR
• Gene ID: 25237
• mRNA Refseq: NM_022441
• Protein Refseq: NP_071886