Active Recombinant Rat Acvrl1 protein, His&hFc-tagged

• Cat.No.: Acvrl1-245R
• Product Overview: Active Recombinant Rat Acvrl1 protein(NP_071886.1)(Met 1-Ala 118) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-118 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 40-200 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
• Molecular Mass: The secreted recombinant rat ACVRL1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 343 amino acids with a predicted molecular mass of 38.7 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Acvrl1 activin A receptor type II-like 1 [ Rattus norvegicus ]
• Official Symbol: Acvrl1
• Synonyms: ACVRL1; activin A receptor type II-like 1; serine/threonine-protein kinase receptor R3; SKR3; TSR-I; Activin receptor likekinase 1; TGF-B superfamily receptor type I; R3; R-3; SETHKIR
• Gene ID: 25237
• mRNA Refseq: NM_022441
• Protein Refseq: NP_071886