Active Recombinant Rat Carboxypeptidase B Protein, Tag Free

• Cat.No.: CPB-01R
• Product Overview: Recombinant Carboxypeptidase B is expressed in E.coli and is purified by high pressure liquid chromatography. No trace contamination with proteases, such as carboxypeptidase A or chymotrypsin, nor protease inhibitors such as PMSF.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Carboxypeptidase B catalyzes the hydrolysis of basic amino acids lysine, arginine, and histidine at the C-terminal end of polypeptides. The molecular weight is 33.8 kDa, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
• Tag: Non
• Form: White to off-white lyophilized powder
• Bio-activity: ≥170 Units/mg protein
• CAS #: 9025-24-5
• Molecular Mass: 34.5 kDa
• Purity: > 95%, Purified by HPLC
• Additives: Tris salts, NaCl and carbohydrates.
• Unit Definition: One unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25 centigrade, pH 7.65.
• Stability: Provided as sterile lyophilized powder, highly stable during transportation and storage.
• Storage: Store in a sealed container at 2-8 centigrade. Once reconstituted, it should be stored at -20 centigrade. The product is stable for 24 months. Greater than 90% enzymatic activity retains after 10 repeated freeze-and-thaw cycles.

Gene Information

• Official Symbol: CPB
• Synonyms: CPB; Carboxypeptidase B