Active Recombinant Rat EPO, His-tagged

• Cat.No.: EPO-578R
• Product Overview: Recombinant Rat EPO (P29676-1) (Met 1-Arg 192), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect cells
• Species: Rat
• Tag: His
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human EPOR-Fc in a functional ELISA.
  2. Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA.
  3. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA.
  4. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA.
  5. Measured by its ability to inhibit Epo-dependent proliferation of TF1 human erythroleukemic cells.
  The ED50 for this effect is 2-10ng/mL.
• Molecular Mass: The recombinant rat EPO comprises 176 amino acids with and has a predicted molecular mass of 20 kDa. It migrates as an approximately 27 kDa band in reduced SDS-PAGE.
• Protein length: Met 1-Arg 192
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epo erythropoietin [ Rattus norvegicus ]
• Official Symbol: EPO
• Gene ID: 24335
• mRNA Refseq: NM_017001.1
• Protein Refseq: NP_058697.1

Reviews

12/28/2021

Stability is truly remarkable, can maintain its activity even under extreme conditions.

08/31/2021

Can catalyze reactions even under extreme conditions.

02/03/2019

This protein's repeatability is truly exceptional, consistently delivering reliable results even under challenging conditions.

Q&As

What is the relationship between EPO protein and cardiovascular disease?

EPO protein can promote the renewal and repair of heart blood vessels, and has the effect of protecting the heart from ischemia-reperfusion damage.

How does EPO protein regulate erythropoiesis?

By binding to receptors, EPO proteins activate signaling pathways that promote the proliferation and differentiation of hematopoietic stem cells in bone marrow into erythrocytes.

How about the clinical use of EPO protein?

EPO protein can be used by injection or oral, and the specific method and dosage need to be determined according to the condition and the doctor's advice.

What are the implications of EPO protein research for the future of treating anemia?

The study of EPO protein helps to understand the regulatory mechanism of erythropoiesis and provides new ideas and methods for the treatment of anemia, such as developing more effective EPO protein analogues or improving existing treatment regimens.

How is EPO protein treated for anemia?

This protein stimulates the bone marrow to produce more red blood cells, thereby increasing hemoglobin levels and treating anemia.

What is the role of EPO protein in kidney disease?

In kidney disease, due to impaired kidney function, the production of EPO protein decreases, leading to anemia. Supplementation with exogenous EPO protein can help correct anemia caused by kidney problems.