Active Recombinant Rat EPO, His-tagged

• Cat.No.: EPO-578R
• Product Overview: Recombinant Rat EPO (P29676-1) (Met 1-Arg 192), fused with a polyhistidine tag at the C-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Rat
• Source: Insect cells
• Tag: His
• Protein Length: Met 1-Arg 192
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human EPOR-Fc in a functional ELISA.
  2. Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA.
  3. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA.
  4. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA.
  5. Measured by its ability to inhibit Epo-dependent proliferation of TF1 human erythroleukemic cells.
  The ED50 for this effect is 2-10ng/mL.
• Molecular Mass: The recombinant rat EPO comprises 176 amino acids with and has a predicted molecular mass of 20 kDa. It migrates as an approximately 27 kDa band in reduced SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epo erythropoietin [ Rattus norvegicus ]
• Official Symbol: EPO
• Gene ID: 24335
• mRNA Refseq: NM_017001.1
• Protein Refseq: NP_058697.1