Active Recombinant Rat FCGR2B protein, His-tagged

• Cat.No.: FCGR2B-1439R
• Product Overview: Recombinant Rat FCGR2B protein(His32-Pro212), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 32-212 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: GMP Monoclonal Anti-Human CD3 Antibody (OKT3) immobilized on CM5 Chip can bind Rat CD32b Protein, His Tag with an affinity constant of 1.93 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 22.4 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: His32-Pro212
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr2b Fc fragment of IgG, low affinity IIb, receptor (CD32) [ Rattus norvegicus ]
• Official Symbol: FCGR2B
• Synonyms: FCGR2B; Fc fragment of IgG, low affinity IIb, receptor (CD32); low affinity immunoglobulin gamma Fc region receptor II-b; FC-gamma RII; igG Fc receptor II beta; Fc receptor, IgG, low affinity IIb; low affinity immunoglobulin gamma FC region receptor II; FCRII; Fcgr2
• Gene ID: 289211
• mRNA Refseq: NM_175756
• Protein Refseq: NP_786932