Source : |
HEK293 |
Species : |
Rat |
Tag : |
His |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl,pH 7.5 with trehalose as protectant. |
Bio-activity : |
Immobilized Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag with a linear range of 0.2-13 ng/mL.Immobilized Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Vitronectin, Tag Free, primary amine labeling with a linear range of 0.1-3 ng/mL. |
Molecular Mass : |
Rat ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, has a calculated MW of 113 kDa (ITGAV) & 77 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. |
Proteinlength : |
Phe31-Pro988&Gly22-Ser679 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |