| Description : |
Thymus Chemokine-1, also called Chemokine (C-X-C motif) ligand 7 (CXCL7), is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, Thymus Chemokine-1 has been shown to bind CXCR-2 and be a chemoattractant forneutrophils and play a role in their activation. Although CTAP-III, β-TG and PBP represent amino-terminal extended variants of Thymus Chemokine-1 and possess the same CXC chemokine domains, these proteins do not exhibit Thymus Chemokine-1 activity. Recently, it has been shown that the additional amino-terminal residues of CTAP-III mask the critical ELR receptor binding domain that is exposed on Thymus Chemokine-1 and may account for lack of Thymus Chemokine-1 activity. Rat CXCL7 shares 72% amino acid sequence identity with mouse CXCL7. |
| Source : |
CHO |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
The EC50 value of rat Thymus Chemokine-1/CXCL7 on Ca^2+ mobilization assay in CHO-K1/Gα15/rCXCR2 cells (human Gα15 and rat CXCR2 stably expressed in CHO-K1 cells) is less than 300 ng/mL. |
| Molecular Mass : |
9.8 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
62 |
| AA Sequence : |
IELRCRCTNTLSGIPLNSISRVNVF RPGAHCDNVEVIATLKNGKEVCLDP TAPMIKKIVKKI |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 97% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant Rat Thymus Chemokine-1/CXCL7 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Rat Thymus Chemokine-1/CXCL7 should be stable up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
