Active Recombinant Rat VTCN1 protein, His-tagged

• Cat.No.: VTCN1-6550R
• Product Overview: Recombinant Rat VTCN1 protein( Phe 29 - Gly 257 ), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat B7-H4, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 27.1 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Phe 29 - Gly 257
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Vtcn1 V-set domain containing T cell activation inhibitor 1 [ Rattus norvegicus ]
• Official Symbol: VTCN1
• Synonyms: VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; immune costimulatory protein B7-H4; RGD1311204
• Gene ID: 295322
• mRNA Refseq: NM_001024244
• Protein Refseq: NP_001019415